Search Search

Home

 

MISSION

The Beckman Institute CLOVER Center’s mission is to facilitate optogenetic studies, custom viral vector development, and tissue clearing projects across Caltech through infrastructure and reagent sharing, training, and further technology and methodology development. By providing these services, the Beckman Institute CLOVER Center is catalyzing high-impact and high-risk research projects by helping researchers test their hypotheses and obtain the preliminary data necessary to secure additional funding for continued technological development or to advance basic science objectives. 

 

TECHNOLOGY

         

 

Tissue Clearing

   

AAV Vector Development

   

 

Opsin Engineering

We are developing improved methods for imaging through thick intact tissue samples at high resolution by rendering them optically transparent and permeable to stains, antibodies and nucleic acid probes.

   

We are developing custom recombinant adeno-associated viruses (AAVs) for more efficient and targeted gene transfer in vivo.                                                                                         

   

Optogenetics uses light sensitive proteins called opsins to modulate and monitor the activity of neurons and other cellular states. We are working to extend the properties of opsins through protein engineering.

 

INFRASTRUCTURE / RESOURCES

  • The CLOVER Center is equipped with a BSL-2 virus production facility as well as wet labs for molecular cloning, tissue processing, and macromolecular staining procedures.

  • CLOVER offers researchers on-site use of tissue clearing facilities and technical support.

  • CLOVER offers collaborative custom vector development.

 

TRAINING / WORKSHOPS

  • One-on-one consultations and training for tissue clearing techniques and viral vector production to jump start research projects

  • Focused workshops for researchers with various backgrounds and experience levels

  • Yearly 5-day course on systemic AAVs and CLARITY as part of the NeuroNex MINT Technology Hub

 

FUNDING